Cloning independent site-directed mutagenesis using total RNA as template
نویسندگان
چکیده
منابع مشابه
Cloning independent site-directed mutagenesis using total RNA as template.
Several site-directed mutagenesis methods have been developed and are being currently used in research. All these methods apply three or four primers, including one or two bearing the appropriate mutation, and most use PCR to amplify the desired sequence and to incorporate the mutation (1–6). Although several developments have improved the robustness and reliability of the method, all of them r...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2004
ISSN: 0736-6205,1940-9818
DOI: 10.2144/04365bm02